Occurrence of Three Major Soybean Viruses, Soybean mosaic virus, Soybean yellow mottle mosaic virus and Soybean yellow common mosaic virus Revealed by a Nationwide Survey of Subsistence Farming Soybean Fields

Research in Plant Disease 2013;19(4):319-325.
Published online December 30, 2013.

영세농가 콩 재배지의 Soybean mosaic virus, Soybean yellow mottle mosaic virus 및 Soybean yellow common mosaic virus 병 발생 조사

조승희 ( Seung Hee Cho ) , 김정규 ( Jung Kyu Kim ) , 이미가 ( Mei Jia Li ) , 서은영 ( Eun Young Seo ) , 임승모 ( Seung Mo Lim ) , 홍석명 ( Seok Myeong Hong ) , 문제선 ( Jae Sun Moon ) , ( John Hammond ) , 임현섭 ( Hyoun Sub Lim )

Occurrence of Three Major Soybean Viruses, Soybean mosaic virus, Soybean yellow mottle mosaic virus and Soybean yellow common mosaic virus Revealed by a Nationwide Survey of Subsistence Farming Soybean Fields

Seunghee Cho1, Jungkyu Kim1, Meijia Li1, Eunyoung Seo1, Seungmo Lim2,3, Seok Myeong Hong4, Jae Sun Moon2,3, John Hammond5 and Hyoun-Sub Lim1*

¹Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea
²Greenbio Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Korea
³Department of Biosystems and Bioengineering, University of Science and Technology, Daejeon 305-350, Korea
⁴GenoRevo Korea Co., Ltd., Suwon 441-853, Korea
⁵United States Department of Agriculture-Agrocultural Research Service, Floral and Nursery Plants Research Unit, Beltsville, Maryland, 20705, USA

Received: October 07, 2013 Revised: October 29, 2013 Accepted: November 04, 2013

Abstract

Soybean yellow mottle mosaic virus (SYMMV) and Soybean yellow common mosaic virus (SYCMV) were recently isolated in Korea, and it has not been reported how two viruses were dispersed in Korea. In 2012, we performed nationwide survey in subsistence soybean farming. Suspicious virus-infected infected leave were collected from the field and a total of 682 soybean tissue samples were assayed by RT-PCR using triplex primers detecting SYMMV, SYCMV, and Soybean mosaic virus (SMV). On hundred two samples showed SMV positive, and SYMMV and SYCMV were detected in 116 and 17 tissue samples, respectively. No sample showed double infection of SYMMV and SYCMV, but there were double infection tissues indicating two viruses positive of SMV plus SYMMV (5 tissue samples) and SMV plus SYCMV (1 tissue sample). Through this first subsistence soybean farming field survey, we assumed soybean viruses were originated from home seed production managed by farmer. Thus, in order to prevent possible seed transmission and further damage caused by virus transmission, virus-free commercial soybean seeds are recommended to be planted.

Key Words: Double infection, Soybean field, Soybean mosaic virus, Soybean yellow common mosaic virus, Soybean yellow mottle mosaic virus