Inhibitory Effects of Resveratrol and Piceid against Pathogens of Rice Plant, and Disease Resistance Assay of Transgenic Rice Plant Transformed with Stilbene Synthase Gene

Research in Plant Disease 2013;19(3):177-182.
Published online September 30, 2013.

Sang-Mi Yu1, Ha Kyung Lee1, Ui-Seon Jeong1, So Hyeon Baek2, Tae-Hwan Noh2, Soon Jong Kwon3 and Yong Hoon Lee1,4*

¹Division of Biotechnology, Chonbuk National University, Iksan 570-752, Korea
²Department of Rice and Winter Cereal Crop, National Institute of Crop Science, Rural Development Administration, Iksan 570-080, Korea
³Department of Agricultural Biotechnology, National Academy of Agricultural Sceince, Rural Development Administration, Suwon 441-707, Korea
⁴Advanced Institute of Environment and Bioscience, and Plant Medical Research Center, Chonbuk National University, Korea

Received: June 23, 2013 Revised: June 11, 2013 Accepted: July 13, 2013

Abstract

Resvestrol has been known to inhibit bacterial and fungal growth in vitro, and can be accumulated in plant to concentrations necessary to inhibit microbial pathogens. Hence, stilbene synthase gene has been used to transform to synthesize resveratrol in heterologous plant species to enhance resistance against pathogens. In the present study, we investigated the antimicrobial activities of resveratrol and piceid to bacterial and fungal pathogens, which causing severe damages to rice plants. In addition, disease resistance was compared between transgenic rice varieties, Iksan 515 and Iksan 526 transformed with stlibene synthase gene and nontransgenic rice varieties, Dongjin and Nampyeong. Minimum inhibitory concentration of resveratrol for Burkolderia glumae was 437.5 μM, and the mycelial growth of Biplaris oryzae was slightly inhibited at concentration of 10 μM. However, other bacterial and fungal pathogens are not inhibited by resveratrol and piceid. The expression of the stilbene synthase gene in Iksan 515 and Iksan 526 did not significantly enhanced resistance against bacterial grain rot, bacterial leaf blight, sheath blight, and leaf blight. This study is the first report on the effect of resveratrol and piceid against pathogens of rice plant, and changes of disease resistance of transgenic rice plants transformed with stilbene synthase gene.

Key Words: Iksan 526, Piceid, Resveratrol, Stilbene synthase, Transgenic rice