Detection of <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> in Soil on the Basis of PCR Amplification

Research in Plant Disease 2004;10(4):310-312.
Published online December 31, 2004.

PCR을 통한 토양에서 Pseudomonas syringae pv. actinidiae의 검출

한효심, 고영진¹, 정재성^*

순천대학교 생물학과, ¹응용생물학과

Detection of Pseudomonas syringae pv. actinidiae in Soil on the Basis of PCR Amplification

Hyo-Shim Han, Young-Jin Koh1, Jae-Sung Jung*

Department of Biology and ¹Department of Applied Biology, Sunchon National University, Sunchon 540-742, Korea

Correspondence: Jae-Sung Jung,Phone)+82-61-750-3616, Fax)+82-61-750-3608, E-mail)jjung@sunchon.ac.kr

Received: November 18, 2004

Abstract

Pseudomonas syringae pv. actinidiae is the causative agent of bacterial canker in kiwifruit. A nested PCR detection method that uses primers designed from the cfl gene, involved in production of the phytotoxin coronatine, was applied on soil samples. These primers yielded 665 and 310-bp fragments in consecutive PCR amplification step with DNA from soil inoculated with Korean strain of P. syringae pv. actinidiae. This system was applied to survey soil samples from a kiwifruit orchard destroyed by bacterial canker. A specific 310-bp PCR product was obtained from all six samples of soil tested.

Key Words: cfl gene, nested PCR, Pseudomonas syringae pv. actinidiae, Soil