Research in Plant Disease 2004;10(4):310-312.
Published online December 31, 2004.
PCR을 통한 토양에서 Pseudomonas syringae pv. actinidiae의 검출
한효심, 고영진1, 정재성*
순천대학교 생물학과, 1응용생물학과
 
Detection of Pseudomonas syringae pv. actinidiae in Soil on the Basis of PCR Amplification
Hyo-Shim Han, Young-Jin Koh1, Jae-Sung Jung*
Department of Biology and 1Department of Applied Biology, Sunchon National University, Sunchon 540-742, Korea
Correspondence:  Jae-Sung Jung,Phone)+82-61-750-3616, Fax)+82-61-750-3608, E-mail)jjung@sunchon.ac.kr
Received: November 04, 2004
Abstract
Pseudomonas syringae pv. actinidiae is the causative agent of bacterial canker in kiwifruit. A nested PCR detection method that uses primers designed from the cfl gene, involved in production of the phytotoxin coronatine, was applied on soil samples. These primers yielded 665 and 310-bp fragments in consecutive PCR amplification step with DNA from soil inoculated with Korean strain of P. syringae pv. actinidiae. This system was applied to survey soil samples from a kiwifruit orchard destroyed by bacterial canker. A specific 310-bp PCR product was obtained from all six samples of soil tested.
Key Words: cfl gene, nested PCR, Pseudomonas syringae pv. actinidiae, Soil


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