| 강낭콩 종자에서 Pseudomonas savastanoi pv. phaseolicola의 검출을 위한 PCR 프라이머의 개발 |
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조정희, 정민정, 송민지, 임규옥, 이혁인, 김정희, 백지현, 차재순 |
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| Development of PCR Primers to Detect Pseudomonas savastanoi pv. phaseolicola from the Bean Seeds |
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Jung Hee Cho, Min Jung Jeong, Min Ji Song, Kyu Ock Yim, Hyok In Lee, Jung Hee Kim, Ji Hyun Baeg, Jae Soon Cha |
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| Abstract |
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PCR primers were developed to detect Pseudomonas savastanoi pv. phaseolicola, a causal agent of halo blight that occurs in all species of common bean (Phaseolus vulgaris L.), from the bean seeds. A primer set, Psp-JHF and Psp-JH-R, specifically amplified 513 bp fragment from Pseudomonas savastanoi pv. phaseolicola only. A nested primer set, psp-JH-F-ne and psp-JH-R-ne, designed from the 1st PCR amplicon, amplified 169 bp fragment. The primer sets did not amplify any non-specific DNA from the seed extracts of Fabaceae including 4 beans, 2 soybeans, and 2 peas. The detection sensitivity of the nested PCR method developed in this study was much higher than that of ELISA and selective medium. PCR assays developed in this study should be useful to detect Pseudomonas savastanoi pv. phasolicola from the bean seeds. |
| Key Words:
Halo blight pathogen, PCR assays, Pseudomonas savastanoi pv. phaseolicola, Seed detection |
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