Immunocapture RT-PCR for Detection of Seed-borne Viruses on Cucurbitaceae Crops

Lee, Hyok In; Kim, Jung Hee; Yea, Mi Chi

Research in Plant Disease 2010;16(2):121-124.
Published online August 30, 2010.

Immunocapture RT-PCR을 이용한 박과작물 종자전염 바이러스의 검출

이혁인, 김정희, 예미지

Immunocapture RT-PCR for Detection of Seed-borne Viruses on Cucurbitaceae Crops

Hyok In Lee, Jung Hee Kim, Mi Chi Yea

Abstract

Immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) was applied to the detection of Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), and Zucchini green mottle mosaic virus (ZGMMV) on Cucurbitaceae crops. These seed-borne tobamoviruses were accurately detected from the infected leaves and seeds by IC-RT-PCR. This method was estimated to be about 100 times more sensitive than ELISA, and also it allowed the direct confirmation of ELISA results by using the captured antigens from a completed ELISA microwell. This convenient and reliable method could be used routinely for large-scale field surveys or seed tests of Cucurbitaceae crops.

Key Words: CGMMV, IC-RT-PCR, KGMMV, ZGMMV