Research in Plant Disease 2008;14(3):233-237.
Published online December 30, 2008.
우리나라 토마토에 발생한 토마토황화잎말림바이러스(Tomato yellow Leaf curl geminivirus)의 초간편 Virion Capture(VC)/PCR 진단법
조점덕, 김태성, 김주희, 최국선, 정봉남, 최홍수, 김정수
 
Convenient Virion Capture (VC)/PCR for Tomato yellow Leaf curl geminivirus Occurring on Tomato in Korea
Jeom Deog Cho, Tae Seong Kim, Ju Hee Kim, Gug Seoun Choi, Bong Nam Chung, Hong Soo Choi, Jeong Soo Kim
Abstract
Tomato yellow leaf curl virus (TYLCV), a newly reported Geminivirus from tomato, generated recently large economic losses in Korea. Development of a fast and precise genetic diagnosis technique for detecting TYLCV which Agricultural research and extension services can utilize easy and handy is very important to prevent yield losses. Virion Capture (VC)/PCR is a simple, accurate and economical genetic detection method without any works or commercial kits for the extraction of the nucleic acid from the infected plants. Primers of twenty two for detection of TYLCV were designed and tested with extracted total DNA or crude sap from tomato leaf infected with TYLCV and healthy plant. Nine primers for total DNA using conventional PCR and another 9 primers for VC/PCR were selected eventually. Primers of six having same specificity were selected from the two methods and tested with other Geminivirus, Tobacco leaf curl virus (TLCV) by VC/PCR. Finally specific primers of four were selected for detection of TYLCV using VC/PCR, and Deng (540, 541), a degenerate primer for Geminivirus reported in 1996, was also developed for VC/PCR.
Key Words: Tomato, Tomato yellow Leaf curl virus, Virion Capture (VC)/PCR
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