중복기생균 Ampelomyces quisqualis 94013의 배양적 특성 |
이상엽, 류재당, 김홍기 |
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Cultural Characteristics of a Hyperparasite, Ampelomyces quisqualis 94013 |
Sang Yeob Lee, Jae Dang Ryu, Hong Gi Kim |
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Abstract |
Ampelomyces quisqualis 94013 (AQ94013), a hyperparasite, was selected as an effective biological control agent against cucumber powdery mildew. Optimal temperature for mycelial growth of AQ94013 was 26℃, and the optimal pH was 6.5. Conidia of AQ94013 were more produced on potato dextrose agar (PDA) in darkness than under alternating cycles of 12 hr fluorescent light and 12 hr darkness. Temperature range for spore germination of the fungus was 10~35℃, and optimal temperature was 20℃. Conidial germination of the fungus began 8 hr after incubation at 24℃. Germination rate of conidia at concentration of 5 × 10(5) spores/ml and 5 × 10(6) spores/ml was higher than at concentration of 5 × 10(7) spores/ml. The best source of carbon and nitrogen for mycelial growth of the fungus were dextrin and neopeptone, respectively. |
Key Words:
Ampelomyces quisqualis 94013, Biological control, Cucumber powdery mildew, Hyperparasite, Spore germination |
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