| 충북지역 토마토 시설재배지의 풋마름병균(Ralstonia solanacearum) 오염도 및 분리균주의 특성 |
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윤건식, 박상용, 강효중, 이기열, 차재순 |
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| Contamination Level of Ralstonia solanacearum in Soil of Greenhouses Cultivating Tomato Plants in Chungbuk Province and Characteristics of the Isolates |
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Gon Sig Yun, Sang Yong Park, Hyo Jung Kang, Ki Yeol Lee, Jae Soon Cha |
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| Abstract |
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Contamination level and characteristics of Ralstonia solanacearum in soil of greenhouses cultivating tomato plants in Chungbuk province was determined. R. solanacearum was isolated with the semiselective media in 27 greenhouse soil samples out of 133 greenhouses soil investigated, which indicates 20.3% of tomato cultivating greenhouses in Chungbuk contaminated with the bacterial wilt pathogen. Density of R. solanacearum was estimated to 10(2~4) cfu/g in the contaminated soil. All 71 isolates of R. solanacearum which containing 12 isolates from the diseased tomato plants were race 1, and 35 isolates of them were biovar 3 and 36 isolates were biovar 4. More than 88% of 71 isolates were inhibited growth on nutrient agar containing oxolinic acid 0.5 μg/ ml, streptomycin 25 μg/ml, tetracycline 5 μg/ml and cupric sulfate 375 μg/ml (1.5 mM). The 11.3%, 4.2% and 5.6% of the isolates can grow on nutrient agar containing 10 times more oxolinic acid, streptomycin, tetracycline than minimal inhibitory concentration of the sensitive strains. Five isolates were resistant to 2 bactericides and one isolates was resistant to all 3 bactericides. |
| Key Words:
Bactericide resistance, Biovar, Contamination Level, Race, Ralstonia solanacearum, Tomato plants |
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