Research in Plant Disease 2003;9(3):107-115.
Published online September 30, 2003.
한국산 장마(Dioscorea opposita cv. Jang-Ma)에서 분리한 Chinese yam necrotic mosaic virus
강동균, 곤도투루, 신종희, 신혜영, 성정현, 강상구, 장무웅
 
Chinese yam necrotic mosaic virus Isolated from Chinese Yam in Korea
Dong Kyoon Kang, To Ru Kondo, Jong Hee Shin, Hye Young Shin, Jung Hyun Sung, Sang Gu Kang, Moo Ung Chang
Abstract
Chinese yam (Dioscorea opposita cv. Jang-Ma) plants showing necrotic mosaic symptom were collected from their growing fields in Andong, Euisong, Gunwi and Daegu, Korea. Direct negative staining method by electron microscope showed filamentous particles of about 660 nm in length. Immunosorbent electron microscopy (ISEM) revealed filamentous particles of 660 nm decorated with antiserum of Chinese yam necrotic mosaic virus (ChYNMV). The virues purified partially were used to isolate viral RNA as template for RT-PCR to amplify about 1.2 kbp of 3`-terminal region (coat protein, 3`-UTR) with ChYNMV specific and oligo-dT primers. Amino acids sequences of amplified CP genes revealed that the viruses shared 97.9% similarity with ChYNMV (AB044386) which was known as the member of Macluravirus. So the viruses from Chinese yam (D. opposita cv. Jang-Ma) plants were identified as ChYNMV. Comparing the CP amino acid sequences of ChYNMV strains with other macluraviruses such as Cardamon mosaic virus (CdMV), Narcissus latent virus (NLV) and Maclura mosaic virus (MacMV) revealed that N-terminal was the most variable region and conserved regions were present within the genus Macluravirus.
Key Words: Chinese yam necrotic mosaic virus, coat protein gene, Dioscorea opposita, Macluravirus


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