Characterization of Tobacco rattle virus(TRV-K) isolated in Korea

Shin, Hye Young; Koo, Bong Jin; Kang, Sang Gu; Chang, Moo Ung; Ryu, Ki Hyun

Research in Plant Disease 2002;8(4):207-214.
Published online December 30, 2002.

한국에서 분리한 Tobacco rattle virus(TRV-K)의 특성

신혜영, 구봉진, 강상구, 장무웅, 류기현

Characterization of Tobacco rattle virus(TRV-K) isolated in Korea

Hye Young Shin, Bong Jin Koo, Sang Gu Kang, Moo Ung Chang, Ki Hyun Ryu

Abstract

Tobacco rattle virus(TRV) was detected from Gladiolus hybridus, Crocus spp. and Narcissus spp. leaves showing notched or stripe on the leaf and malformation symptoms collected from Daegu and Kyungbuk province by electron microscopy (EM), immunosorbent electron microscopy (ISEM) and host range study. Direct negative staining method by EM showed rigid rod long particles 170~200 × 22 nm and rigid rod short particles 40~114 × 22 nm. TRV-K isolated from G. hybridus propagated with Nicotiana tabacum. TRV coat protein(CP) gene was amplified using specific oligonucleotide primer by RT-PCR. Sequence analysis of amplified CP gene showed 99.5% nucleotide similarity to TRV-ORY.

Key Words: CP-gene sequence, RT-PCR, Tobravirus, Tobacco rattle virus