Research in Plant Disease 2014;20(4):307-313.
Published online December 15, 2014.
국내 브루그만시아에서 분리한 Brugmansia mosaic virus의 특성
박충열1 · 김봉섭1 · 남문1 · 이민아1 · 백다솜1 · 배양수1 · 박은혜1 · 김정선3 · 최종윤4 · 임승모5,6 · 문제선5,6 · 이수헌1,2*
1경북대학교 응용생명과학부, 2경북대학교 식물의학연구소, 3국립농업과학원 농업미생물과, 4국립특작과학원 사과시험장, 5과학기술연합대학원대학교(UST) 시스템생명공학과, 6한국생명공학연구원 식물시스템공학연구센터
 
Characterization of Brugmansia mosaic virus Isolated from Brugmansia spp. in Korea
Chung Youl Park1, Bong-Sub Kim1, Moon Nam1, Min-A Lee1, Da-Som Baek1, Yang Su Bae1, Eun-Hye Park1, Jeong-Sun Kim3, Jong-Yoon Choi4, Seungmo Lim5,6, Jae Sun Moon5,6 and Su-Heon Lee1,2*
1School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Korea
2Institute of Plant Medicine, Kyungpook National University, Daegu 702-701, Korea
3Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Wanju 565-851, Korea
4Apple Research Station, National Institute of Horticultural & Herbal Science, Gunwi 716-812, Korea
5Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon 305-350, Korea
6Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea
Correspondence:  <p>Su-Heon Lee</p><p>Tel : +82-5,

Su-Heon Lee

Tel : +82-53-950-5763
Fax: +82-53-950-6758
E-mail: suheon@knu.ac.kr

Received: October 14, 2014   Revised: November 14, 2014   Accepted: December 14, 2014
Abstract

In May 2013, an angel’s trumpet leaves showing mosaic and malformation symptoms were collected from Suwon city, Gyeonggi-do. An analysis of the collected sample by transmission electron microscopy observation showed filamentous rod particles of 720-800 nm in length. On the basis of the these observations, we performed PCR against three reported Potyviruses (Brugmansia mosaic virus, Colombian datura virus and Brugmansia suaveolens mottle virus), and the sample was positive for BruMV. Pathogenicity and host range test of BruMV was determined by mechanical inoculation. Solanaceae (tobacco, tomato and eggplant) and Amaranthaceae (ground cherry) appeared typical virus symptoms. To determine coat protein of this virus, we designed specific primer pairs, and performed PCR amplification, cloning, and sequencing. Phylogenetic analysis showed that BruMV-SW was most closely related to BruMV isolate SK. Comparison of the BruMV-SW coat protein nucleotide sequences showed 92% to 99% identities to the other BruMV isolates.

Key Words: Angel’s trumpet, Bioassay, Brugmansia, Potyvirus


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