Research in Plant Disease 2014;20(4):270-274.
Published online December 15, 2014.
Raffaelea quercus-mongolicae와 Raffaelea spp. 인공접종에 의한 신갈나무 줄기에서의 병원성 평가
손수연 · 서상태* · 박형철
국립산림과학원 산림병해충연구과
 
Assessment of Virulence of Raffaelea quercus-mongolicae and Raffaelea spp. Isolates by Artificial Inoculation of Quercus mongolica Logs
Su-Yeon Son, Sang-Tae Seo* and Hyung-Cheol Park
Division of Forest Diseases & Insect Pests, Korea Forest Research Institute, Seoul 130-712, Korea
Correspondence:  <p>Sang-Tae Seo</p><p>Tel : +82-,

Sang-Tae Seo

Tel : +82-2-961-2667
Fax: +82-2-961-2679
E-mail: stseo@forest.go.kr

Received: August 12, 2014   Revised: October 07, 2014   Accepted: October 31, 2014
Abstract
The objective of this study was to evaluate the virulence of geographically different isolates of oak wilt pathogen, Raffaelea quercus-mongolicae and other Raffaelea species. In this study, mature trees of Quercus mongolica were inoculated with the various isolates of Raffaelea spp. and their virulence was evaluated by measuring the extent of sapwood discoloration resulting from the inoculation. The average length of discolored sapwood in a lateral direction was longest in the trees inoculated with the isolates from Korea (8.69 cm) followed by R. quercivora (7.51 cm) and the other Raffaelea spp. (3.35 cm). The lateral length of discolored sapwood caused by the inoculation with Korean strains varied from 4.71 to 14.90 cm indicating their differences in virulence. The area of discolored sapwood caused by the inoculation with Raffaelea spp. varied from 1.57 to 8.42 cm2 indicating their differences in virulence. Based on the length and area of the discolored sapwoods, isolated YY and wj43 appeared to have the highest virulence among all the Raffaelea isolates tested. Each of the two isolates was obtained from Gangwon Province and Jeonbuk Province, respectively.
Key Words: Inoculation, Oak wilt, Raffaelea quercus-mongolicae
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