Evaluation of Cabbage- and Broccoli-genetic Resources for Resistance to Clubroot and Fusarium Wilt

Research in Plant Disease 2014;20(4):235-244.
Published online December 15, 2014.

뿌리혹병 및 시들음병에 대한 저항성 양배추와 브로콜리 유전자원 탐색

이지현¹ · 조은주¹ · 장경수¹ · 최용호¹ · 김진철² · 최경자¹*

¹한국화학연구원 바이오화학연구센터, ²전남대학교 응용생물공학부

Evaluation of Cabbage- and Broccoli-genetic Resources for Resistance to Clubroot and Fusarium Wilt

Ji Hyun Lee1, Eun Ju Jo1, Kyoung Soo Jang1, Yong Ho Choi1, Jin-Cheol Kim2 and Gyung Ja Choi1*

¹Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology, Daejeon 305-600, Korea
²Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture, Chonnam National niversity, Gwangju 500-757, Korea

Correspondence: Gyung Ja Choi,

Gyung Ja Choi

Tel : +82-42-860-7434
Fax: +82-42-861-4913
E-mail: kjchoi@krict.re.kr

Received: October 21, 2014 Revised: December 01, 2014 Accepted: December 04, 2014

Abstract

Clubroot and Fusarium wilt of cole crops (Brassica oleracea L.) are destructive diseases which for many years has brought a decline in quality and large losses in yields all over the world. The breeding of resistant cultivars is an effective approach to reduce the use of chemical fungicides and minimize crop losses. This study was conducted to evaluate the resistance of 60 cabbage (B. oleracea var. capitata) and 6 broccoli (B. oleracea var. italica) lines provided by The RDA-Genebank Information Center to clubroot and Fusarium wilt. To investigate resistance to clubroot, seedlings of the genetic resources were inoculated with Plasmodiophora brassicae by drenching the roots with a mixed spore suspension (1 : 1) of two isolates. Of the tested genetic resources, four cabbage lines were moderately resistant and ‘K166220’ represented the highest resistance to P. brassicae. The others were susceptible to clubroot. On the other hand, to select resistant plants to Fusarium wilt, the genetic resources were inoculated with Fusarium oxysporum f. sp. conglutinans by dipping the roots in spore suspension of the fungus. Among them, 17 cabbage and 5 broccoli lines were resistant, 16 cabbage lines were moderately resistant, and the others were susceptible to Fusarium wilt. Especially, three cabbage (‘IT227115’, ‘K161791’, ‘K173350’) and two broccoli (‘IT227100’, ‘IT227099’) lines were highly resistant to the fungus. We suggest that the resistant genetic resources can be used as a basic material for resistant B. oleracea breeding system against clubroot and Fusarium wilt.

Key Words: Breeding, Crucifer, Disease resistance, Fusarium oxysporum f. sp. conglutinans, Plasmodiophora brassicae