Research in Plant Disease 2013;19(2):124-127.
Published online June 30, 2013.
파프리카 과실에 괴사반점을 일으키는 Pepper mild mottle virus의 병원형
최국선 ( Gug Seoun Choi ) , 최승국 ( Sung Kook Choi ) , 조점덕 ( Jeom Deog Cho ) , 조인숙 ( In Sook Cho )
 
A Pathotype of Pepper mild mottle virus Causing Necrotic Spot Symptoms in Paprika Fruit
Gug-Seoun Choi*, Sung-Kook Choi, Jeom-Deog Cho and In-Sook Cho
Horticulture and Herbal Environmental Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon 441-440, Korea
Received: February 19, 2013   Revised: April 01, 2013   Accepted: June 09, 2013
Abstract
Black necrotic spots were observed from the fruits of paprika that were cultivating in a vinylhouse. The casual agents of the symptom were identified as several isolates of Pepper mild mottle virus (PMMoV) by responses of indicator plants, electron microscopy, and RT-PCR analysis. Symptoms of the viral disease were mild mottle in the young leaves, necrotic spots on the fruits and the fruit apex of paprika, but the symptoms were not shown on the mature leaves. All of the PMMoV isolates were determined as P1.2.3 pathotypes from the biological responses on the chilli pepper lines used for discrimination of tobamovirus pathotypes. Pathogenicity of the PMMoV isolates was also confirmed using mechanical inoculation method to paprika seedlings. The coat protein (CP) genes of the PMMoV isolates were compared at the nucleotide and amino acid levels with the previously published PMMoV isolate. The isolates share 96 to 99% CP nucleotide identity among the isolates. The CP of P1.2-pathotype PMMoV-P2 presented Met at position 139, But the CPs of P1.2.3- pathotype PMMoVs from paprika showed Met to Asn substitution at the same position. This is the first report of identification of P1.2.3-pathotype PMMoV isolates from paprika in Korea.
Key Words: Coat protein, Identification, Paprika, Pathotype, Pepper mild mottle virus, PMMoV


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