| 보문 : RT-PCR에 의한 카네이션괴저바이러스와 카네이션둥근반점바이러스 정밀진단 |
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이시원 ( Si Won Lee ) , 강은하 ( Eun Ha Kang ) , 허노열 ( Noh Yeol Heo ) , 김상목 ( Sang Mok Kim ) , 김유정 ( Yu Jeong Kim ) , 신용길 ( Yong Gil Shin ) |
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| Detection of Carnation necrotic fleck virus and Carnation ringspot virus Using RT-PCR |
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Siwon Lee1,2, Eun-Ha Kang1,3, Noh-Yeol Heo1, Sang-Mok Kim1, Yu-Jeong Kim1 and Yong-Gil Shin1* |
1Plant Quarantine Technology Center, Suwon 443-440, Korea
2Department of Microbiology, Dankook University, Cheonan 330-714, Korea
3Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Korea |
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Received: June 14, 2012 Revised: February 13, 2013 Accepted: February 20, 2013 |
| Abstract |
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Carnation is considered to be one of the top three cutting flowers in the world, which is a main crop with 21 billion annual volume of manufacture. The four carnation items such as cuttings, seed, plant and unrooted cuttings are imported and exported. Viruses can be easily transmitted during vegetative propagation of carnation. Carnation necrotic fleck virus (CNFV) and Carnation ringspot virus (CRSV) are designated as Korea plant quarantine viruses and inspected. This study was aimed to develop specific primer sets for easy and rapid detection of CNFV and CRSV. Two RT-PCR primer sets were efficiently amplified 288 and 447 bp fragments for CNFV and 503 549 bp fragments for CRSV. Furthermore, developed nested primer sets make possible to high sensitive detection and verification. CNFV nested PCR primer sets all produced band of 147 bp and CRSV nested PCR primer sets did bands of 395 and 347 bp. In addition, plasmid inserted 6 sequences in amplicon were used as a positive control to improve inspection confidence. The successful application of PCR module newly developed in this study will be highly useful for detect of CNFV and CRSV for quarantine inspections. |
| Key Words:
Carnation, Carnation necrotic fleck virus, Carnation ringspot virus, Mutation-positive control |
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