Research in Plant Disease 2012;18(4):298-303.
Published online December 30, 2012.
초고속 Real-time PCR을 이용한 Tomato yellow Leaf curl virus의 신속진단
김택수 ( Tack Soo Kim ) , 최승국 ( Seung Kook Choi ) , 고민정 ( Min Jung Ko ) , 이민호 ( Min Ho Lee ) , 최형석 ( Hyung Seok Choi ) , 이세원 ( Se Weon Lee ) , 박경석 ( Kyung Seok Park ) , 박진우 ( Jin Woo Park )
 
Ultra-rapid Real-time PCR for the Detection of Tomato yellow leaf curl virus
Tack-Soo Kim, Seung-Kook Choi1, Min-jung Ko, Minho Lee2, Hyung seok Choi, Se-Weon Lee, Kyungseok Park and Jin-Woo Park*
Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-440, Korea
1Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon 441-440, Korea
2Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-440, Korea
Received: November 08, 2012   Revised: November 23, 2012   Accepted: November 30, 2012
Abstract
Tomato yellow leaf curl virus (TYLCV), transmitted exclusively by the whitefly (Bemisia tabaci) in a circulative manner is one of the most important virus in tomato. Since the first report of TYLCV incidence in Korea in 2008, the virus has rapidly spread nationwide. TYLCV currently causes serious economic losses in tomato production in Korea. Early detection of TYLCV is one of the most important methods to allow rouging of infected tomato plants to minimize the spread of TYLCV disease. We have developed an ultrarapid and sensitive real-time polymerase chain reaction (PCR) using a new designed real-time PCR system, GenSpectorTM TMC-1000 that is a small and portable real-time PCR machine requiring only a 5 μl reaction volume on microchips. The new system provides ultra-high speed reaction (30 cycles in less than 15 minutes) and melting curve analysis for amplified TYLCV products. These results suggest that the short reaction time and ultra sensitivity of the GenSpectorTM-based real-time PCR technique is suitable for monitoring epidemics and pre-pandemic TYLCV disease. This is the first report for plant virus detection using an ultra-rapid realtime PCR system.
Key Words: Tomato yellow leaf curl virus (TYLCV), Ultra-rapid real-time PCR


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