| Burkholderia gladioli pv. alliicola CH1의 병원성 및 Polygaractronase Isozymes 생성 |
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이찬중 ( Chan Jung Lee ) , 이종태 ( Jong Tae Lee ) , 김영태 ( Yeong Tae Kim ) , 전창성 ( Chang Sung Jhune ) , 정종천 ( Jong Chun Cheong ) , 박완 ( Wan Park ) |
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| Phytopathogenicity of Burkholderia gladioli pv. alliicola CH1 and Production of PGase Isozymes |
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Chan Jung Lee*, Jong Tae Lee1, Yeong Tae Kim2, Chang Sung Jhune, Jong Chun Cheong and Wan Park3 |
Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Suwon 441-707, Korea
1Onion Research Institute, Gyeongnam Agricultural Research and Extension Services, Changnyeong 635-821, Korea
2Genomics Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Korea
3School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Korea |
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Received: October 05, 2011 Revised: February 20, 2012 Accepted: September 24, 2012 |
| Abstract |
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Burkholderia gladioli pv. alliicola CH1 showed typical soft rot symptoms at higher than 20oC but very weak soft rot symptoms at temperature under 10oC. Among the nine agro-chemicals, oxolinic acid WP, streptomycin + copper hydroxide WP and streptomycin WP were found to be effective for the inhibition of the pathogen in vitro. The results of scanning electron microscopic investigation showed that onion bulbs was macerated by infection of B. gladioli pv. alliicola CH1. B. gladioli pv. alliicola CH1 was able to produce polygalacturonase but did not produce pectin lyase and carboxymethylcellulase. In analysis of the polygalacturonase activity of the isolated pectin-degradation enzymes from B. gladioli pv. alliicola CH1 total protein, three activity bands 45 kDa, 35 kDa, and 29 kDa were detected by the direct (or in-gel) activity staining on SDS-PAGE. |
| Key Words:
Burkholderia gladioli pv. alliicola, Polygaractronase, SDS-PAGE |
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