Detection of Xanthomonas axonopodis pv. aurantifolii and Xanthomonas axonopodis pv. citrumelo by Triplex PCR

Research in Plant Disease 2012;18(2):129-132.
Published online June 30, 2012.

Sang-Mi Yu1, Se Won Lee2, Seungdon Lee2, Eun Woo Park3 and Yong Hoon Lee1,4*

¹Division of Biotechnology, Chonbuk National University, Iksan 570-752, Korea
²Crop Protection Division, National Academy of Agricultural Science, RDA, Suwon 441-707, Korea
³Dept. of Applied Biology and Chemistry, Seoul National University, Seoul 151-921, Korea
⁴Advanced Institute of Environment and Bioscience, and Plant Medical Research Center, Chonbuk National University, Korea

Received: November 09, 2011 Revised: March 21, 2011 Accepted: May 29, 2012

Abstract

Citrus bacterial canker is an economically important disease affecting citrus production in many citrusgrowing areas and several pathotypes have been recognized within the Xanthomonas pathogens causing canker. In view of the containment of the disease, accurate identification of the causal bacterium is important. In this study, triplex PCR method was developed by using the previously reported primers. Two groups of primer combination, such as, one group including primers 2/3, J-pth1/J-pth2 and XACF/XACR, and another group 2/3, J-pth1/J-pth2 and Xac01/Xac02, were suitable for the detection and differentiation of X. a. pv. citri Aw, X. a. pv. aurantifolii B and C, and X. a. pv. citrumelo E strains. Moreover, the primer combination of Xac01 and J-pth2 promised us to use as a specific primer set to detect X. a. pv. citrumelo E strain. The PCR methods developed in this study could be used for the rapid differentiation of Xanthomonas pathotypes of citrus.

Key Words: Citrus, Diversity, Pathotype, Xanthomonas axonopodis