Biological Control of Botrytis Leaf Blight of Lily and Botrytis Gray Mold of Cucumber by Ulocladium atrum

Research in Plant Disease 2004;10(4):319-323.
Published online December 31, 2004.

Ulocladium atrum을 이용한 백합 잎마름병 및 오이 잿빛곰팡이병의 생물학적 방제

이남영, 권은미, 김진철¹, 유승헌^*

충남대학교 농업생명과학대학 농생물학과, ¹한국화학연구원 생물기능연구팀

Biological Control of Botrytis Leaf Blight of Lily and Botrytis Gray Mold of Cucumber by Ulocladium atrum

Nam-Young Lee, Eun-Mi Kwon, Jin-Cheol Kim1, Seung-Hun Yu*

Department of Agricultural Biology, College of Agriculture and Life Sciences, Chungnam National University, 305-764, Korea
¹Biological Function Research Team, Korea Research Institute of Chemical Technology, Daejeon 305-606, Korea

Correspondence: Seung-Hun Yu,Phone)+82-42-821-5762, Fax)+82-42-823-8679, E-mail)shunyu@cnu.ac.kr

Received: November 09, 2004

Abstract

This study was conducted to investigate the effect of U. atrum treatment on control of Botrytis leaf blight of lily and Botrytis gray mold of cucumber, and to evaluate the U. atrum as the biological control agent of Botrytis diseases. The antagonistic isolates CNU 9037 and CNU 9054 isolated from tomato leaves were identified as Ulocladium atrum Preuss based on morphological characteristics. This is the first record of U. atrum in Korea. In bioassays on dead leaves of tomato and cucumber, treatment of U. atrum colonized the dead leaves and suppressed sporulation of Botrytis as compared with the untreated control. The suppression of spoulation of Botrytis on dead leaf segments by U. atrum was higher when U. atrum was treated before Botrytis was treated. The effect of treatments with conidial suspension of U. atrum on leaf blight of lily and gray mold of cucumber caused by Botrytis elliptica and B. cinerea, respectively, was investigated under greenhouse conditions. Spraying U. atrum (1 × 10⁶ per ml) at intervals of 1 week for three times resulted in a significant reduction of natural infections of lily leaves caused by B. elliptica. Protective value of U. atrum treatment was higher than that of the fungicide (procymidone) treatment. Spraying U. atrum also resulted in a significant reduction of cucumber gray mold caused by B. cinerea. Our results show that U. atrum has a potential for biological control against diseases caused by Botrytis spp, in lily and cucumber.

Key Words: Biological control, Botrytis cinerea, Botrytis elliptica, Ulocladium atrum