Comparative Assay Fungus Population and Resistant Genes about <i>Magnaporthe grisea</i> between Korea and China

Research in Plant Disease 2004;10(4):217-226.
Published online December 31, 2004.

한국·중국 벼 도열병균 생리형 분석 및 품종 저항성 특성 비교

김동윤¹, 심홍식¹, Lei Cailin², Zhong Zhuan Ling², 한성숙^*

작물과학원 환경생명공학과, ¹농업과학기술원 농업생물부 식물병리과, ²중국농업과학원 작물육종재배연구소

Comparative Assay Fungus Population and Resistant Genes about Magnaporthe grisea between Korea and China

Dong-Yun Kim1, Hong-Sik Shim1, Seong Ho Choi, Lei Cailin2, Zhong Zhuan Ling2, Seong-Sook Han*

Crop Environment and Biotechnology Div. National Institute of Crop Science, RDA, Suwon 441-857, Korea
¹Plant Pathology Division, National Institute of Agricultural Sciences & Technology, Suwon 441-707, Korea
²Rice Research Division, Institute of Crop Breeding and Cultivation, CAAS, Beijing 100081, China

Correspondence: Lei Cailin,Phone)+82-31-290-6758, Fax)+82-31-290-6752, E-mail)sshan@rda.go.kr

Received: November 09, 2004

Abstract

One hundred isolates of Magnaporthe grisea from Korea and China were characterized for pathogenicity using eight Korean differential varieties(KDV), six Chinese differential varieties(CDV) and six near isogenic lines(NILs) developed in China. The restriction length polymorphism of M. grisea isolates from each country also was analyzed using MGR586 as a probe. One hundred Korean isolates classified into 17 races on KDV were grouped into 29 pathotypes on Chinese near isogenic lines(NILs). Virulence of 46% of Korean isolates against all the six Chinese NILs indicated that the current six Chinese NILs alone was not enough to be used as differential varieties in Korea. Especially, susceptibility of the BL1 carrying resistance gene Pi-b to 70% of tested Korean isolates suggested that BL1(Pi-b) may not be a useful resistance source to Korean blast. Based on the virulence assays of M. grisea populations from each country were divided into two groups. About 50% of Chinese isolates showed similarity to the 30% of the Korean isolates. Especially, the isolates from northern part of China, where Japonica rice cultivars were grown, showed high similarity to the Korean isolates, while isolates from southern part of China, where Indica rice were mainly grown, showed low similarity to Korean isolates. The genome RFLPs of Korean isolates were quite different from those of southern part of China using MGR586 as a probe. These data indicated that the physiological and genetical characteristics of M. grisea population might be determined by strong interaction with cultivated rice.

Key Words: China, Korea, Resistant gene, RFLP, Rice blast