| 종 특이 primer를 이용한 옥수수 오염 Fusarium verticillioides의 PCR 검출 |
|
강미란, 김지혜, 이승호, 류재기, 이데레사, 윤성환 |
| |
| Detection of Fusarium verticillioides Contaminated in Corn Using a New Species-specific Primer |
|
Mi Ran Kang, Ji Hye Kim, Seung Ho Lee, Jae Gee Ryu, Theresa Lee, Sung Hwan Yun |
|
|
| Abstract |
|
Fusarium verticillioides (teleomorph: Gibberella moniliformis), a member of the Gibberellea fujikuroi species complex, causes rots of corn stalks and ears, and produces a group of mycotoxins known as fumonisins that are harmful to animals and humans. Here, we focus on the development of a species-specific PCR primer set for differentiating F. verticillioides from other fumonisin-producing Fusarium species belonging to the species complex, such as F. proliferatum, F. fujikuroi, and F. subglutinans that are frequently associated with corn. The specific primers (RVERT1 and RVERT2) derived from the nucleotide sequences of RNA polymerase II beta subunit (RPB2) gene amplified a 208 bp-DNA fragment from only F. verticillioides isolates among the potential fumonisin-producing species examined; all of these isolates were shown to carry FUM1 required for fumonisin biosynthesis. The PCR detection limit using this specific primer set was approximately 0.125 pg/μl genomic DNA of F. verticillioides. In addition, the F. verticillioides-specfic fragment was successfully amplified from genomic DNAs of corn samples contaminated with Fusarium spp. This primer set would provide a useful tool for the detection and differentiation of potential fumonisin-producing F. verticillioides strains in cereal samples. |
| Key Words:
Corn ear and stalk rot, Gibberella, Fumonisin, RNA polymerase II subunit |
|
PDF Links
Download Citation
