Research in Plant Disease 2011;17(2):169-176.
Published online August 30, 2011.
PCR-SSCP 분석에 의한 Phytophthora katsurae의 분자생물학적 특성
이선근, 장하나, 이동현, 이상현, 이상용, 이종규
 
Molecular Characteristics of Phytophthora katsurae Using PCR-SSCP Analysis
Sun Keun Lee, Ha Na Jang, Dong Hyeon Lee, Sang Hyun Lee, Sang Yong Lee, Jong Kyu Lee
Abstract
Phytophthora katsurae is the fungus responsible for chestnut ink disease. The objectives of this study were to determine if a single-strand conformation polymorphism (SSCP) analysis of rDNA-ITS region, elongation factor 1 alpha gene and β-tubulin gene could be used for rapid identification and genetic diversity of P. katsurae, and to assess the potential use of the SSCP technique as a diagnostic tool for P. katsurae. Each regions amplified by PCR using primers designed to overlap the genus Phytophthora were characterized for the Phytophthora species. PCR products were denatured and electrophoresed for SSCP analysis. P. katsurae isolates showed an unique pattern in SSCP analysis and were easily distinguished from other Phytophthora species used as the control. This indicates that SSCP analysis is an useful technique for distinguishing Phytophthora species from genetically close relatives, and show that the SSCP analysis of each region is an efficient detection tool for P. katsurae. But PCR-SSCP analysis of single-gene may have difficulty in distinguishing P. katsurae from other Phytophthora species. Therefore, PCR-SSCP analysis of multi-genes can be useful for rapid and effective identification of P. katsurae.
Key Words: Genetic diversity, Identification, PCR-SSCP analysis, Phytophthora katsurae


ABOUT
BROWSE ARTICLES
EDITORIAL POLICY
FOR CONTRIBUTORS
Editorial Office
Rm,904 (New Bldg.) The Korean Science & Technology Center 22, Teheran-ro 7-gil, Gangnam-gu, Seoul 06130, Korea
Tel: +82-2-557-9360    Fax: +82-2-557-9361    E-mail: paper@kspp.org                

Copyright © 2024 by The Korean Society of Plant Pathology.

Developed in M2PI

Close layer
prev next