Research in Plant Disease 2010;16(3):247-253.
Published online December 30, 2010.
Apple Scar Skin viroid 발생상황 및 Real-time RT-PCR을 이용한 상대정량 분석
김대현, 김현란, 허성, 김세희, 김민아, 신일섭, 김정희, 조강희, 황정환
Occurrence of Apple Scar Skin viroid and Relative Quantity Analysis Using Real-time RT-PCR
Dae Hyun Kim, Hyun Ran Kim, Seong Heo, Se Hee Kim, Min A Kim, Il Sheob Shin, Jeong Hee Kim, Kang Hee Cho, Jeong Hwan Hwang
Apple scar skin viroid (ASSVd) is one of the smallest viral pathogens infecting fruits, especially apple, and causes a significant damage to fruit trees. ASSVd usually induced the skin-dapple ring symptoms, but in ``Fuji`` varieties, corked spot were occurred on the fruit skin in 2009. This new symptom will be of great helpful to diagnosis ASSVd in sight. ASSVd was surveyed in apple and pear from 2009 to 2010 in Korea, and ASSVd was identified in 20 out of 1,193 trees. The infection rate was 1.7%. To screen the infectivity of ASSVd among apple cultivars, real-time RT-PCR was applied followed by designing of ASSVd specific primers based on highly conserved regions of several ASSVd isolates including Korean isolate. NADH dehydrogenase subunit 5 (nad 5) gene, which is mRNA of the mitochondrial gene, was used for internal control. In this study, ASSVd infected apples were classified into 12 groups depending on different symptoms and symptom severity (scaring, rusting or malformation). Taken together, this study suggested that real-time PCR analysis was more sensitive to detect the low copy of ASSVd on early viroid infected apple skins than regular RT-PCR method.
Key Words: Apple scar skin viroid, Occurrence, Relative quantity analysis, Real-time RT-PCR
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