Research in Plant Disease 2010;16(2):125-128.
Published online August 30, 2010.
자두 검은점무늬병원균의 PCR진단 및 검출
류영현, 이중환, 권태영, 김승한, 김동근
 
PCR Primer Developed for Diagnosis of Xanthomonas arboricola pv. pruni in Prune
Young Hyun Ryu, Joong Hwan Lee, Tae Young Kwon, Seung Han Kim, Dong Geun Kim
Abstract
Bacterial black spot disease of prune fruit (Prunus salicina cv. formosa) has outbroke around major prune production area, Gimcheon, Euiseong and Gunwi in Gyungbuk province and has caused severe economic loss. Integrons PCR primer was designed along with sample pre-incubation and nested PCR method to enhance detection sensitivity for early detection of bacteria in fields. Designed integrons PCR primer successfully detected Xanthomonas arboricola pv. pruni from field collected samples, fruit, leaf, branch and even in raindrop collected from prune orchard. Pre-incubation along with nested PCR enhanced sensitivity to detect X. arboricola pv. pruni from seemingly healthy looking, symptomless branches. Designed integrons PCR can be used in prune nursery fields and in plant quarantine practice for the detection of X. arboricola pv.pruni.
Key Words: Integrons, Nested PCR, PCR detection, Prunus, Xanthomonas arboricola pv. pruni
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