| 길항세균 Bacillus amyloliquefaciens A-2를 이용한 토마토 잎곰팡이병 방제용 미생물 제제 |
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공현기, 전옥주, 최기혁, 이광렬, 백정우, 김현주, 센틸쿠마무루가이얀, 문병주, 이선우 |
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| Formulation of Bacillus amyloliquefaciens A-2 and Its Efficacy to Control Tomato Leaf Mold Caused by Fulvia fulva |
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Hyun Gi Kong, Ock Joo Chun, Ki Hyuck Choi, Kwang Youll Lee, Joung Woo Baek, Hyun Ju Kim, Senthilkumar Murugaiyan, Byung Ju Moon, Seon Woo Lee |
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| Abstract |
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This study was performed to develop a formulation using an antagonistic bacterium Bacillus amyloliquefaciens A-2 to control tomato leaf mold caused by Fulvia fulva. B. amyloliquefaciens A-2 was grown in a medium with rice oil and mixed with various carrier and additives. One of the formulations, A2-MP, showed the best disease control value among the tested formulations. The disease control value of A2-MP at 100-fold and 500- fold diluted treatment was not significantly different from that of chemical fungicide triflumizole in a growth chamber. Although disease control effect was decreased by serial diluted treatment of the prepared A2-MP, 1,000-fold diluted treatment of A2-MP still showed high disease control value of 72.0%. For the green house experiments, the disease control values of A2-MP was indicated as 79.4% which is similar to that of chemical fungicide, triflumizole showing 79.6%. When the disease control activity of the formulation A2-MP was compared in tomato production conditions, disease control values of 100-fold diluted A2-MP and 3,000 fold diluted triflumizole exhibited 60%, 81.6%, respectively. The disease control efficiency by A-2MP was 73% of the disease control value of chemical fungicide. The formulation A-2MP maintained the stable bacterial viability and disease control activity when stored at 4oC. This result suggested that A-2MP develped from B. amyloliquefaciens A-2 could be used to control tomato leaf mold. |
| Key Words:
Bacillus amyloliquefaciens, Biocontrol formulation, Fulvia fulva, Tomato Leaf mold |
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