Characterization of Rice black-streaked dwarf virus in Maize

Lee, Bong Choon; Yoon, Young Nam; Hong, Sung Jun; Hong, Yeon Kyu; Hwang, Jae Bok; Song, Sek Bo; Kang, Hwang Won; Lee, Key Woon

Research in Plant Disease 2008;14(3):223-225.
Published online December 30, 2008.

옥수수에 발생하는 벼검은줄오갈병의 유전자 비교

이봉춘, 윤영남, 홍성준, 홍연규, 황재복, 송석보, 강항원, 이기운

Characterization of Rice black-streaked dwarf virus in Maize

Bong Choon Lee, Young Nam Yoon, Sung Jun Hong, Yeon Kyu Hong, Jae Bok Hwang, Sek Bo Song, Hwang Won Kang, Key Woon Lee

Abstract

This study was carried out to identify the Rice black-streaked dwarf virus that infected maize plants collected from Gochang-gun in Jeollabukdo in 2005. The genomic dsRNA from infected plants was extracted and the genome pattern was analyzed by polyacrylamide gel electrophoresis. Results of the electrophoresis revealed the already known 10-segment genome and the difference of mobility was confirmed in isolates by collected areas. The RBSDV was identified from the result of RT-PCR using the template of extracted dsRNA and specific primer. The results of S10 cloned to pGEM-T vector and the conducted in sequence analysis consisted of 1,801nt and 559aa. This was of the same size as the RBSDV S10 identified in rice, and the change was confirmed in 18 base and displayed homology of 99%.

Key Words: Genomic dsRNA, Nucleotide sequence, Rice black-streaked dwarf virus