| 석량풋콩재배지에서의 동부모자이크바이러스병의 발생도 및 풋콩병해모니터링과 동부모자이크 바이러스의 순화 |
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조의규 |
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| Severity of Cowpea mosaic virus and Putkong Disease Monitoring and Purification of Cowpea mosaic virus |
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Eui Kyoo Cho |
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| Abstract |
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One hundred and eighty-six leaves of soybean cv. Seokryangputkong that showed mild mosaic symptoms were collected randomly and ELISA tests were conducted with those leaf samples to screen the presence of Cowpea mosaic virus (CPMV). Ninety-three out of 186 samples reacted positively to CPMV, but those samples did negatively to Soybean mosaic virus (SMV). At least, 55 leaf samples revealed higher values than that of positive control. The results strongly confirmed that CPMV occurred severely in soybean cv. Seokryangputkong. However, a question is raised on the primary reservoir and vector for transmission of this virus. Since the farmer changes seeds every year, seed transmission is excluded. The virus was also purified, the analysis of coat protein conformed the virus of cowpea mosaic virus and UV absorption pattern confirmed that the causal virus of mosaic disease in soybean putkong was cowpea mosaic virus. |
| Key Words:
Soybean cv. "Seokryangputkong", ELISA, Cowpea mosaic virus, CPMV, Soybean mosaic virus, SMV |
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