Tobamoviruses of Green Peppers Growing on Hydroponic Systems

Choi, Gug Seoun; Kim, Jae Hyun; Kim, Jeong Soo; Kim, Hyun Ran

Research in Plant Disease 2004;10(3):194-197.
Published online September 30, 2004.

풋고추 수경재배에서 발생하는 tobamovirus의 특성

최국선, 김재현, 김정수, 김현란

Tobamoviruses of Green Peppers Growing on Hydroponic Systems

Gug Seoun Choi, Jae Hyun Kim, Jeong Soo Kim, Hyun Ran Kim

Abstract

Incidence of tobamovirus diseases was 100% at late growth stage of green pepper on hydroponic systems in plastic house. Infection frequency of the diseases showed 34% of Pepper mild mottle virus (PMMoV), 41.5% of Tobacco mild green mottle virus (TMGMV), and 24.5% of the co-infected viruses. The two viruses specifically reacted in DAS-ELISA prepared with each polyclonal antibody. A total of 77 pure tobamovirus isolates obtained from the crops was tested for pathotype determination. The isolation frequency of tobamovirus pathotype P0 and P1,2 was 61% and 39%, respectively. All TMGMV isolates belonged to the pathotype P0. In restriction enzyme analysis of the cDNAs synthesized with coat protein gene of PMMoV pathotype P0 and P1,2, the former had two TaqI sites but the later had one.

Key Words: hydroponic system, pathotype, pepper, PMMoV, TMGMV, tobamovirus