Detection of Rice Stripe Virus using RT-PCR

Lee, Bong Choon; Hong, Yeon Kyu; Kwak, Do Yeon; Oh, Byeong Geun; Park, Sung Tae; Kim, Soon Chul

Research in Plant Disease 2004;10(1):30-33.
Published online March 30, 2004.

RT-PCR에 의한 벼 줄무뉘잎마름병 정밀진단

이봉춘, 홍연규, 곽도연, 오병근, 박성태, 김순철

Detection of Rice Stripe Virus using RT-PCR

Bong Choon Lee, Yeon Kyu Hong, Do Yeon Kwak, Byeong Geun Oh, Sung Tae Park, Soon Chul Kim

Abstract

Until now, occurrence of rice stripe virus (RSV) is limitted in southern part of Korea. However, recently the occurrence of RSV is increased and spreaded in central part of Korea including Chungcheong and Kyonggi province. It is very difficult to distinguish RSV symptoms on virus symptom physiological damage of rice. We detected RSV viral RNA from infected rice and its insect vector Laodelphax striatellus using specific primer of RSV-polymerase and coat protein gene with reverse transcription (RT)-PCR. The result of RT-PCR, we observed specific band including RSV-polymerase (1,023 bp) and CP (969 bp) in both host of rice and insect vector.

Key Words: Laodelphax striatellus, RSV, RT-PCR