| 단보 : Corynespora cassiicola에 의한 로즈마리 점무늬병 |
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이왕휴 ( Wang Hyu Lee ) , 한상준 ( Sang Jun Han ) , 최인영 ( In Young Choi ) |
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| Occurrence of Target Spot on Rosemary Caused by Corynespora cassiicola in Korea |
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Wang-Hyu Lee1*, Sang-Jun Han1 and In-Young Choi2 |
1Department of Agricultural Biology, Chonbuk National University, Jeonju 561-765, Korea (Plant Medical Research Center: Industry, Academy, Research, Cooperation Company for Herb, Jeollabuk-do)
2Jeollabuk-do Agricultural Research and Extension Services, Iksan 570-704, Korea |
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Received: October 04, 2012 Revised: January 21, 2013 Accepted: February 02, 2013 |
| Abstract |
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The purpose of this experiment was to investigate the development of new spot disease on the leaf and stem of rosemary (Rosmarinus officinalis) in commercial greenhouses at Jeonju and Namwon in Korea. Incidence of target spot on rosemary was higher at the end of the rainy season with high humidity. Those symptoms were black ring spots (3?5 mm in diameter) and withering on green leaves and stems. Conidiophores and conidia were formed on the infected tissue in moist chamber and conidia were shown as the cylindrical and oval types in chain, ranged from 55 to 275 μm in length, and 7 to 14 μm in width. Conidia with eight to ten pseudosepta were formed on the conidiapore. The optimum growth temperature of isolates was 30oC on the PDA medium under the dark condition. In the pathogenesis test, the target spot and withering symptoms were appeared on the leaves and stems 3 days after inoculation showing similar symptoms compared to those of in nature. The same fungus was re-isolated from infected lesion, indicating that Corynespora cassiicola caused leaf target spot and twig blight on rosemary. The rDNA ITS nucleotide sequences of the pure cultured isolate from the diseased area on rosemary showed 100% similarity to the sequences of C. cassiicola available in the GenBank database (JQ595296, JQ595297, FJ852715 and AY238606). Therefore, we report that the target spot of leaves and stems in rosemary was caused by C. cassiicola. |
| Key Words:
Fungal disease, Rosmarinus officinalis, Target spot |
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