Occurrence of Stone Fruit Viruses on Peach Trees (Prunus persica L. Batsch) in Korea

Research in Plant Disease 2012;18(4):391-395.
Published online December 30, 2012.

국내에서 발생하는 복숭아 바이러스병

조인숙 ( In Sook Cho ) , 조점덕 ( Jeom Doeg Cho ) , 최승국 ( Seung Kook Choi ) , 최국선 ( Gug Seoun Choi )

Occurrence of Stone Fruit Viruses on Peach Trees (Prunus persica L. Batsch) in Korea

In Sook Cho*, Jeom Doeg Cho, Seung Kook Choi and Gug Seoun Choi

Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Suwon 441-440, Korea

Received: October 11, 2012 Revised: November 09, 2012 Accepted: November 14, 2012

Abstract

To investigate the occurrence of viruses in peach, leaf samples were collected from peach trees in commercial orchard of six areas in Korea. Reverse transcription polymerase chain reaction (RT-PCR) was used to identify the presence of the following stone fruit viruses: Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV) and Plum pox virus (PPV). About 65.0% of the 515 samples were infected with ACLSV and PNRSV. Virus-like symptoms showing mosaic on leaves was observed in ACLSV infected peach trees. However, PNRSV infected peach trees showed no symptoms. These viral DNAs by sequence analysis were confirmed 4 ACLSV isolates and 3 PNRSV isolates. The Korean peach isolates of ACLSV and PNRSV showed 70?99% and 88?99% amino acid sequence identities, respectively, with those reported previously and their amino acid sequence identities with each other were approximately 95% and 88%, respectively. Phylogenetic analysis indicated that the Korean ACLSV isolates belong to the A group of ACLSV. The Korean PNRSV isolates reported in this study were grouped into I (PV32), II (PV96) and III (PE5) groups.

Key Words: ACLSV, PNRSV, Peach tree (Prunus persica L. Batsch), Sequence analysis