감자 역병의 친환경 방제를 위한 키토산 제형의 살포 |
장태현 ( Tae Hyun Chang ) , 김병섭 ( Byung Sup Kim ) |
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Application of Chitosan Preparations for Eco-friendly Control of Potato Late Blight |
Taehyun Chang* and Byung Sup Kim |
Division of Ecology and Environmental System, College of Ecology & Enviromental Sciences, Kyungpook National University, Sangju 742-711, Korea 1Department of Applied Plant Science, Kangnung Wonju National University, Gangneung 210-702, Korea |
Received: June 29, 2012 Revised: November 20, 2012 Accepted: November 30, 2012 |
Abstract |
Potato late blight caused by Phytophthora infestans Cooke is one of the major diseases in the cultivation of potatoes in Korea. Effect of chitosan preparations (SH-1 and SH-2) was evaluated on the inhibition of mycelial growth of P. infestans, and protective activity using detached potato leaf assay both in vivo and in the field test. SH-1 and SH-2 were showed protective activity of young plant with control values more than 95% potato late blight by inoculation with pathogens under growth chamber conditions. Mycelial growth was inhibited the radial growth over 74% at a concentration of 300 μg/ml of both SH-1 and SH-2. Spraying with SH-1 and SH-2 on the leaves for detached leaf assay reduced disease development. The content of total polyphenol in stem was significantly increased by SH-1 and SH-2 application in the field. In field experiments, foliar application with both SH-1 and SH-2 were significantly reduced the development of late blight on potato plants. Control of late blight disease was obtained with control values of 72% and 53% by application of 1% SH-1 and SH-2, respectively, with 4 times at 7 days interval, and reduced with similar disease control values by application with 3 times at 14 days interval compared with untreated control. SH-1 and SH-2 applications increased the fresh weight of potato, and higher grade potatoes were also increased. The results showed that SH-1 and SH-2 applications can be used as eco-friendly natural fungicide for organic farming for the increase of yields and control of late blight. |
Key Words:
Chitosan, Disease development, Late blight, Organic farming, Phytophthora |
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