Genotypic Characterization of Cherry Witches` Broom Pathogen Taphrina wiesneri Strains

Seo, Sang Tae; Jeong, Su Jee; Lee, Seung Kyu; Kim, Kyung Hee

Research in Plant Disease 2011;17(1):99-101.
Published online April 30, 2011.

벚나무 빗자루병균 Taphrina wiesneri의 유전적 특성

서상태, 정수지, 이승규, 김경희

Genotypic Characterization of Cherry Witches` Broom Pathogen Taphrina wiesneri Strains

Sang Tae Seo, Su Jee Jeong, Seung Kyu Lee, Kyung Hee Kim

Abstract

The ascomycetous fungus Taphrina wiesneri, the pathogen of cherry witches` broom, is highly pathogenic to Prunus yedoensis, the most widely planted cherry trees in Korea as park and roadside trees. A collection of 13 strains of the pathogen in Korea and Japan was characterized by 18S rDNA gene sequence and restriction fragment length polymorphism (RFLP) analysis. In cluster analysis based on 18S rDNA gene sequence the strains were divided into 2 clusters. In RFLP analysis of the rDNA-IGS region using HhaI, the strains were separated into four patterns, B, C, D and G, of which pattern G was new.

Key Words: 18S rDNA, RFLP, Taphrina wiesneri

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