Characterization of Prunus necrotic ringspot virus Isolate from Peach in Korea

Kim, Hyun Ran; Lee, Sin Ho; Shin, Il Sheob; Kim, Jeong Hee; Cho, Kang Hee; Heo, Seong; Kim, Jeong Soo; Choi, Yong Mun

Research in Plant Disease 2009;15(3):170-174.
Published online December 30, 2009.

국내 복숭아에서 분리한 Prunus necrotic ringspot virus의 특성

김현란, 이신호, 신일섭, 김정희, 조강희, 허성, 김정수, 최용문

Characterization of Prunus necrotic ringspot virus Isolate from Peach in Korea

Hyun Ran Kim, Sin Ho Lee, Il Sheob Shin, Jeong Hee Kim, Kang Hee Cho, Seong Heo, Jeong Soo Kim, Yong Mun Choi

Abstract

In this paper, we report a characterization of Prunus necrotic ringspot virus (PNRSV) isolate. The virus was identified from ``Yumyeong`` peach showing mild mosaic on leaves in commercial orchard of ``Umsung``, Chungbuk province in Korea. The virus isolate produced ringspot symptom on the inoculated cotyledons and systemic mosaic and malformation on the upper leaves of Cucumis sativus. Systemic mottles were appeared in Chenopodium quinoa. When the buds of the virus infected stem were grafted on the healthy young Prunus persica GF305 seedlings, line pattern with mosaic appeared within 3 months. Isometric virus-like particles were found in parenchyma cells and plasmodesmata of C. sativus leaves inoculated mechanically with the virus. The cDNA fragments of PNRSV coat protein (CP) region, approximately 675bp, were synthesized from genomic RNA extracted from virus-infected leaves by RT-PCR using specific primer pairs. Partial nucleotide sequences of the CP regions were determined and analyzed with the known PNRSV. The CP gene of PNRSVKorea isolates showed 93.9~94.7% similarity to the 4 known PNRSV isolates.

Key Words: Ilarvirus, Indicator assay, RT-PCR detection, Peach, PNRSV