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Identification of Cherry green ring mottle virus on Sweet Cherry Trees in Korea
Res. Plant Dis. 2013;19:326-330
Published online December 30, 2013
© 2013 The Korean Society of Plant Pathology.

In-Sook Cho*, Gug-Seoun Choi and Seung-Kook Choi

Horticultural and Herbal Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon 441-440, Korea
Phone) +82-31-290-6237, Fax) +82-31-290-6259 Email) tuat@korea.kr
Received October 11, 2013; Revised November 5, 2013; Accepted November 5, 2013.
cc This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
During the 2012 growing season, 154 leaf samples were collected from sweet cherry trees in Hwaseong, Pyeongtaek, Gyeongju, Kimcheon, Daegu, Yeongju and Eumseong and tested for the presence of Cherry green ring mottle virus (CGRMV). PCR products of the expected size (807 bp) were obtained from 6 samples. The PCR products were cloned and sequenced. The nucleotide sequences of the clones showed over 88% identities to published coat protein sequences of CGRMV isolates in the GenBank database. The sequences of CGRMV isolates, CGR-KO 1?6 shared 98.8 to 99.8% nucleotide and 99.6 to 100% amino acid similarities. Phylogenetic analysis indicated that the Korean CGRMV isolates belong to the group II of CGRMV coat protein genes. The CGRMV infected sweet cherry trees were also tested for Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), Cherry necrotic rusty mottle virus (CNRMV), Cherry mottle leaf virus (CMLV), Cherry rasp leaf virus (CRLV), Cherry leafroll virus (CLRV), Cherry virus A (CVA), Little cherry virus 1 (LChV1), Prune dwarf virus (PDV) and Prunus necrotic ringspot virus (PNRSV) by RT-PCR. All of the tested trees were also infected with ACLSV.
Keywords : CGRMV, RT-PCR, Sequence analysis, Sweet cherry


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