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Physiological, Biochemical and Genetic Characteristics of Ralstonia solanacearum Strains Isolated from Pepper Plants in Korea
Res. Plant Dis. 2013;19:265-272
Published online December 30, 2013
© 2013 The Korean Society of Plant Pathology.

Young Kee Lee1 and Hee Wan Kang2,3*

1Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Korea
2Graduate School of Future Convergence Technology, Hankyong National University, Ansung 456-749, Korea
3Institute of Genetic Engineering, Hankyong National University, Ansung 456-749, Korea
Phone) +82-31-670-5420, Fax) +82-31-676-2602 Email) kanghw2@hknu.ac.kr
Received October 30, 2013; Revised November 12, 2013; Accepted November 19, 2013.
cc This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Totally sixty three bacteria were isolated from lower stems showing symptoms of bacterial wilt on pepper plants in 14 counties of 7 provinces, Korea. The isolates showed strong pathogenicity on red pepper (cv. Daewang) and tomato (cv. Seogwang) seedlings. All virulent bacteria were identified as Ralstonia solanacearum based on colony types, physiological and biochemical tests and polymerase chain reaction (PCR). All R. solanacearum isolates from peppers were race 1. The bacterial isolates consisted of biovar 3 (27%) and biovar 4 (73%). Based on polymorphic PCR bands generated by repetitive sequence (rep-PCR), the 63 R. solanacearum isolates were divided into 12 groups at 70% similarity level. These results will be used as basic materials for resistant breeding program and efficient control against bacterial wilt disease of pepper.
Keywords : Biovar, Classification, Pepper, Ralstonia solanacearum, Rep-PCR


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