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Development of Convenient Screening Method for Resistant Radish to Plasmodiophora brassicae
Res. Plant Dis. 2011;17:161-168
Published online August 30, 2011
© 2011 The Korean Society of Plant Pathology.

조수정 ( Su Jung Jo ) , 장경수 ( Kyoung Soo Jang ) , 최용호 ( Yong Ho Choi ) , 김진철 ( Jin Cheol Kim ) , 최경자 ( Gyung Ja Choi )
cc This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
To establish simple and reliable screening method for resistant radish to Plasmodiophora brassicae Woron. using soil-drenching inoculation, the development of clubroot on radish seedlings inoculated with P. brassicae GN-1 isolate according to several conditions such as inoculum concentration, plant growth stage and incubation period after inoculation was studied. To select resistant radish against clubroot, 10-day-old seedlings were inoculated with P. brassicae by drenching the roots with the spore suspension of the pathogen to give 1×10(9) spores/pot. The inoculated seedlings were incubated in a growth chamber at 20℃ for 3 days then cultivated in a greenhouse (20±5℃) for 6 weeks. Under the optimum conditions, 46 commercial cultivars of radish were tested for resistance to YC-1 (infecting 15 clubroot-resistant cultivars of Chinese cabbage) and GN-1 (wild type) isolates of P. brassicae. Among them, thirty-five cultivars showed resistance to both isolates and one cultivar represented susceptible response to the pathogens. On the other hand, the other cultivars showed different responses against the tested P. brassicae pathogens. The results suggest that this method is an efficient system for screening radish with resistance to clubroot.
Keywords : Breeding, Clubroot, Crucifer crops, Cultivar, Resistance

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