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Characterization of Three Korean Isolates of Malva Vein Clearing Virus from Curled Mallow (Malva verticillata)
Res. Plant Dis. 2020;26:283-288
Published online December 31, 2020
© 2020 The Korean Society of Plant Pathology.

Hae-Ryun Kwak1†, Ji-Gwang Kim2†, Jeong-Eun Kim3, Hyeon-Yong Choi1, Hong-Soo Choi1, and Mikyeong Kim3*

1Crop Protection Division, National Institute of Agricultural Sciences, Wanju 55365, Korea
2Chungcheongnam-do Agricultural Research and Extension Services, Yesan 32418, Korea
3Department of Plant Medicine, Chungbuk National University, Cheongju 28644, Korea
Tel: +82-43-261-2509
Fax: +82-43-261-2552
E-mail: mkim00@chungbuk.ac.kr
ORCID https://orcid.org/0000-0003-3154-8178
These authors contributed equally to this work.
Received August 27, 2020; Revised October 10, 2020; Accepted October 12, 2020.
cc This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
In September 2017, vein clearing and yellowing symptoms resembling those caused by viruses were observed on leaves of Malva verticillata in Chungnam, Korea. Nucleic acids were extracted from leaves of five symptomatic plants and tested by reverse transcription polymerase chain reaction using four virus specific primer pairs including malva vein clearing virus (MVCV). Amplicons of the expected size (600 bp) were obtained from total RNA of all samples using the MVCV-specific primers. To confirm the presence of MVCV in symptomatic plants, the DNA fragments from three samples were purified, and directly sequenced. BLAST analysis revealed that it shared the highest nucleotide identity (99%) with a MVCV isolate from tomato (Mexico). The virus isolates obtained from the third re-inoculated Chenopodium was designated as Cm1–5. Tissue from Cm1, Cm3, and Cm5 isolates was mechanically sap inoculated into 23 indicator plants. Cm3 isolate induced chlorotic local and mosaic symptoms in Althaea rosea. Phylogenetic analysis based on coat protein gene of 19 MVCV isolates from 6 different countries and plant species, did not correlated with either the geographical origin of the isolates, or pathogenicity. To our knowledge, this study first reports the natural occurrence of MVCV on M. verticillata in Korea and characterization of three Korean isolates of MVCV.
Keywords : Curled mallow, Malva, Plant virus disease, Potyvirus


December 2020, 26 (4)