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A Rapid and Simple Detection Assay for Rice Bacterial Leaf Blight by Recombinase Polymerase Amplification
Res. Plant Dis. 2020;26:195-201
Published online December 31, 2020
© 2020 The Korean Society of Plant Pathology.

Shinhwa Kim, Bong Choon Lee, Hyun Ju Kim, Soo Yeon Choi, Su Jwa Seo, and Sang-Min Kim*

Crop Foundation Division, National Institute of Crop Science, Wanju 55365, Korea
Tel: +82-63-238-5344
Fax: +82-63-238-5305
E-mail: kimsm@rda.go.kr
Received March 15, 2020; Revised November 29, 2020; Accepted November 30, 2020.
cc This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Rice bacterial leaf blight (BLB) by Xanthomonas oryzae pv. oryzae (Xoo) is considered to be one of the major rice diseases steadily occurring around the rice-producing countries. In this study, we developed a recombinase polymerase amplification (RPA) assay for the rapid, convenient and specific diagnosis of Xoo by targeting Xoo-specific transposase A gene. As the target gene can be amplified in 10 min without DNA extraction process and special equipment for temperature control, RPA for BLB can be useful and practical component for on-site diagnosis.
Keywords : Bacterial leaf blight, Detection, Recombinase polymerase amplification, Xanthomonas oryzae pv. oryzae


December 2020, 26 (4)