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First Report of a Bracken Blight Disease Caused by Didymella sp.
Res. Plant Dis. 2019;25:143-148
Published online September 30, 2019
© 2019 The Korean Society of Plant Pathology.

Jung Eun Lee1, Ki Beom Kim2, Ju Eun Park2, Da-Woon Kim2, Yoo-Kyoung Shin2, Sung-Hwan Yun2* , and Young-Ryun Chung1*

1Research and Technology Development Institute, JGreen Inc., Jinju 52840, Korea
2Department of Medical Biotechnology, Soonchunhyang University, Asan 31538, Korea
*Y.-R. Chung
Tel: +82-55-761-4701
Fax: +82-55-761-8589
E-mail: yrchung@gnu.ac.kr
*S.-H. Yun
Tel: +82-41-530-1288
Fax: +82-41-530-3085
E-mail: sy14@sch.ac.kr
ORCID
Sung-Hwan Yun
https://orcid.org/0000-0003-3438-6637
Young Ryun Chung
http://orcid.org/0000-0002-4060-1543
Received August 16, 2019; Revised September 11, 2019; Accepted September 11, 2019.
cc This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
An outbreak of new disease with leaf and stem blight symptom occurred at bracken-growing fields in Namhae-gun, Gyeongsangnam-do, Korea during the last 4 years. This new disease caused significant yield losses on bracken production in this area. We have collected diseased leaves and stems showing the blight symptom in May, July, and October 2018 to investigate causal pathogens. A total of 92 fungal isolates were obtained from the diseased samples and their pathogenicity was tested on healthy bracken leaves. From the total isolates, 22 isolates were able to produce the leaf blight symptom similar to the original one found in the fields. To identify two fungal pathogens which showed higher virulence levels compared to other pathogenic isolates, we constructed phylogenetic trees using the nucleotide sequences of genes for ribosomal RNA, RNA polymerase beta subunit, beta tubulin, and internal transcribed region. Most phylogenetic trees constructed indicate that both isolates, which are identical to each other, reside in a clade of the genus Didymella and possibly similar to D. rumicicola or D. acetosellae. Nevertheless, the exact identification of these pathogens at the species level needs further investigations. This is the first report of a blight disease on bracken by Didymella sp.
Keywords : Bracken, Bracken blight disease, Didymella sp.


September 2019, 25 (3)