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Diagnosis of Cylindrocarpon destructans Using Enzyme-Linked Immunosorbent Assay
Res. Plant Dis. 2019;25:131-135
Published online September 30, 2019
© 2019 The Korean Society of Plant Pathology.

Taiying Li1, Sungyeon Ji1, Boknam Jung2, Bo Yeon Kim1, Kwang Sik Lee1, Mun Won Seo3, Sung Woo Lee3, Jungkwan Lee1*, and Seung-Ho Lee3*

1Department of Applied Biology, Dong-A University, Busan 49315, Korea
2Forest Insect Pests and Diseases Division, National Institute of Forest Science, Seoul 02455, Korea
3Ginseng Research Division, National Institute of Horticultural and Herbal Science, Eumseong 27715, Korea
*J. Lee
Tel: +82-51-200-7554
Fax: +82-51-200-7505
E-mail: jungle@dau.ac.kr
*S.-H. Lee
Tel: +82-43-871-5609
E-mail: lsh750409@korea.kr
Received July 11, 2019; Accepted August 30, 2019.
cc This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Cylindrocarpon destructans causes ginseng root rot and produces radicicol that has an antifungal effect. In this study, we developed a method to detect this fungus using enzyme-linked immunosorbent assay (ELISA). Secreted proteins of C. destructans were used as antigens to obtain C. destructans-specific IgG from mouse. Out of 318 monoclonal antibodies generated from mouse, two antibodies (Cd7-2-2 and Cd7-2-10) showed highest specificity and sensitivity. Indirect ELISA using both antigens successfully detected C. destructans in soils, but direct ELISA using IgG conjugated with horseradish peroxidase failed to detect antigens in soils. The indirect ELISA developed here can efficiently detect the fungus and help manage ginseng root rot disease in fields.
Keywords : Cylindrocarpon destructans, Diagnosis, Enzyme-linked immunosorbent assay, Fusarium solani, Ginseng root rot


September 2019, 25 (3)