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Occurrence and Eradication of Plum Pox Virus on Ornamentals in Korea, 2016-2017
Res. Plant Dis. 2019;25:8-15
Published online March 31, 2019
© 2019 The Korean Society of Plant Pathology.

Mikyeong Kim1† , Gi-Su Kim2†, Hae-Ryun Kwak1, Jeong-Eun Kim1, Jang-Kyun Seo3, Seong-Jun Hong4, Gyeong-Jae Lee4, Ju-Hui Kim5, Min-Kyeong Choi5, Byeong-Ryeon Kim6, Ji-Gwang Kim6, In-Yeong Han7, Hyeon-Ju Lee8, Heon-Seop Won9, Hyo-Jung Kang10, Jong-Woo Han10, Suk-Ju Ko11, Hyo-Jeong Kim11, Seung-Han Kim12, Jung-Hywan Lee12, and Hong-Soo Choi1*

1Crop Protection Division, National Institute of Agricultural Science, Wanju 55365, Korea, 2Department of Central Area Crop Science, National Institute of Crop Science, Wanju 55365, Korea, 3Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Korea, 4Extension Service Bureau, Rural Development Administration, Jeongju 54875, Korea, 5Jeollabuk-do Agricultural Research and Extension Services, Iksan 54591, Korea, 6Chungcheongnam-do Agricultural Research and Extension Services, Yesan 32418, Korea, 7Gyeongsangnam-do Agricultural Reasarch and Extension Services, JinJu, 52733, Korea, 8Gyeonggi-do Agricultural Research and Extension Services, Hwasung 18388, Korea, 9Gangwon-do Agricultural Research and Extension Services, Chuncheon 24226, Korea, 10Chungcheongbuk-do Agricultural Research and Extension Services, Boeun 28902, Korea, 11Jeollanam-do Agricultural Research and Extension Services, Naju 58213, Korea, 12Gyeongsangbuk-do Agricultural Research and Extension Services, Daegu 41401, Korea
Tel: +82-63-238-3300, Fax: +82-63-238-3838, E-mail: hschoi@korea.kr, ORCID https://orcid.org/0000-0003-3154-8178
Mikyeong Kim and Gi-Su, Kim have contributed equally to this work.
Received November 17, 2018; Revised February 9, 2019; Accepted March 11, 2019.
cc This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Plum pox virus (PPV) is a significant viral disease in Prunus spp. worldwide. A nationwide survey was started in Prunus spp. orchards, since PPV was first detected from peach in Korea, 2015. During 2016-2017, samples were collected from 30,333 trees in 1,985 orchards of stone fruits in 8 provinces and 4 cities, Korea and tested by RT-PCR using specific PPV primer set. As a result, 21 trees including peach (9 trees), Japanese apricot (4 trees), plum (1 tree), apricot (7 trees) in 10 orchards were infected and controlled by eradication program. Amplicons of the expected size (547 bp) were obtained from total RNA of seven peach trees in 2016, and directly sequenced. BLAST analysis revealed the highest nucleotide (NT) identity (99%) with a PPV D isolates (LC331298, LT600782) in Genbank. The seven isolates from shared nt sequence identities of 98 to 100% with one another. Phylogenetic analysis showed the isolates in peach clustered closely with the PPV-D isolates from Korea, Japan, USA, and Canada. This is, to our knowledge, the first report of the presence of PPV in Prunus spp. orchards in Korea, 2016-2017, we hope that our results and efforts will contribute to effective measures for eradication of PPV.
Keywords : Eradication, Detection, Plum pox virus, Sharka, Stone fruits


March 2019, 25 (1)