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PCR Detection Method for Rapid Diagnosis of Bacterial Canker Caused by Clavibacter michiganensis on Tomato
Res. Plant Dis. 2018;24:342-347
Published online December 31, 2018
© 2018 The Korean Society of Plant Pathology.

Mi-Jeong Park*, Chang-Gi Back, and Jong-Han Park

Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Wanju 55365, Korea
*Tel: +82-63-238-6312 Fax: +82-63-238-6305 E-mail: mijpark@korea.kr
Received October 19, 2018; Revised December 3, 2018; Accepted December 6, 2018.
cc This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Bacterial canker caused by Clavibacter michiganensis is considered to be one of the most serious diseases, leading to economic damage to tomato worldwide. Diagnosis of the bacterial canker on tomato is known to be difficult because the causal pathogen is slow-growing on artificial media as well as causes latent infection in tomato. In this study, as a less time-consuming method, a specific primer set was newly designed for rapid detection of C. michiganensis. The method presented here is so simple, easy, and fast that it can be useful and practical in direct detection of the bacterial canker pathogen from tomato plants.
Keywords : Bacterial canker, Clavibacter michiganensis, Detection, PCR, Tomato


December 2018, 24 (4)