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First Report of Soft Rot by Pectobacterium carotovorum subsp. brasiliense on Amaranth in Korea
Res. Plant Dis. 2018;24:339-341
Published online December 31, 2018
© 2018 The Korean Society of Plant Pathology.

Samnyu Jee*, Jang-Gyu Choi, Suyoung Hong, Young-Gyu Lee, and Min Kwon

Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration, Pyeongchang 25342, Korea
*Tel: +82-33-330-1920 Fax: +82-33-330-1590 E-mail: jsnnimp@korea.kr ORCID https://orcid.org/0000-0001-7834-4303
Received October 13, 2018; Revised November 23, 2018; Accepted December 6, 2018.
cc This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Amaranth has the potential for good materials related to nutrients and health benefits. There are several diseases of amaranth such as leaf blight, damping-off, and root rot. As a causal agent of soft rot disease, Pectobacterium spp. could infect various plant species. In this study, we isolated the bacterial pathogen causing soft rot of amaranth in South Korea. In Gangneung, Gangwon province during 2017, amaranth plants showed typical soft rot symptoms such as wilting, defoliation and odd smell. To isolate pathogen, the macerated tissues of contaminated amaranth were spread onto LB agar plates and purified by a single colony subculture. One ml bacterial suspension of a representative isolate was injected to the stem of five seedlings of 2-week-old amaranth with a needle. Ten mM magnesium sulfate solution was used as a negative control. 16S rDNA gene and recA gene were sequenced and compared with the reference sequences using the BLAST. In the phylogenetic tree based on 16S rDNA gene and recA gene, GSA1 strain was grouped in Pcb.
Keywords : Amaranth, Pcb, Soft rot, South Korea


December 2018, 24 (4)