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Development of a Multiplex RT-PCR for the Simultaneous Detection of Three Viruses in Cherry Plants
Res. Plant Dis. 2018;24:233-236
Published online September 30, 2018
© 2018 The Korean Society of Plant Pathology.

Chung Youl Park1, Jeongran Park1, Geunsik Lee1, Seung-In Yi1, Byeong Hoon Kim2, Jung Sik Eom2, Som Gyeol Lee2, and Hongsup Kim1*

1Seed Testing & Research Center, Korea Seed & Variety Service, Gimcheon 39660, Korea
2Daegu Agricultural Technology Center, Daegu 41159, Korea
Tel: +82-54-912-0241
Fax: +82-54-912-0277
E-mail: hskim98@korea.kr
Received June 28, 2018; Revised July 18, 2018; Accepted July 18, 2018.
cc This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
A multiplex RT-PCR (mRT-PCR) assay was developed for the detection of the recently reported viruses, Cherry virus A (CVA), Little cherry virus 1 (LChV-1), and Little cherry virus 2 (LChV-2), in cherry plants in Korea. Eight sets of primers were designed for each virus and their specificity was tested by using various combinations of mixed primer sets. From the designed primer sets, one combination was selected and further evaluated to estimate the optimum temperature and detection limits of the mRT-PCR. A newly developed mRT-PCR assay was also tested using 20 cherry samples collected in the field. This mRT-PCR assay may be a useful tool for field surveys of diseases and the rapid detection of these three viruses in cherry plants.
Keywords : Cherry virus A, Little cherry virus 1, Little cherry virus 2, Multiplex RT-PCR


September 2018, 24 (3)