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Subspecies Classifying and Characterizing the Two Groups of Antagonistic Sorangium cellulosum against Botrytis cinerea and Colletotrichum acutatum
Res. Plant Dis. 2018;24:213-220
Published online September 30, 2018
© 2018 The Korean Society of Plant Pathology.

Tae-Hoon Koo and Sung-Chul Yun*

Department of Pharmaceutical Engineering and Biotechnology, Sunmoon University, Asan 31460, Korea
Tel: +82-41-530-2282
Fax: +82-41-530-2393
E-mail: scyun@sunmoon.ac.kr
ORCID
https://orcid.org/0000-0001-6295-8642
Received April 30, 2018; Revised May 9, 2018; Accepted May 9, 2018.
cc This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
We classified the previously reported antagonistic strains of Sorangium cellulosum into 5 subspecies (A-E). Four strains were antagonistic to Botrytis cinerea (AB group) and two strains were antagonistic to Colletotrichum acutatum (AC group). According to the genetic and sequential analyses with standard genes, xynB1, bglA2, groEL1 for grouping, all strains of AB group were belonged to subspecies C and all strains of AC group were belonged to subspecies D. In addition, high pressure liquid chromatography with the culture filtrates confirmed the genetic results, because AB group had peaks with retention time at 20-22.5 minutes, whereas AC group had no peak. There was positive relationship (R2=0.9652) between the control values of infecting B. cinerea on cherry tomatoes and the main peak areas of chromatograms among the four isolates of AB group. From the subspecies results of AB group, the main peak of KYC 3270 was expected to be epothilone D. However the retention times of the standard of commercial epothilone D and the main peak of KYC 3270 culture filtrate were different as 9.9 and 11.581 min., respectively. Finally, the antagonistic metabolite of AB group was inferred as 7-ketone epothilone D.
Keywords : 7-ketone epothilone D, Antagonistic metabolites, Chromatography, Myxobacteria, Phytopathogenic fungi


September 2018, 24 (3)