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Development of an Efficient Bioassay Method for Testing Resistance to Bacterial Soft Rot of Radish
Res. Plant Dis. 2018;24:193-201
Published online September 30, 2018
© 2018 The Korean Society of Plant Pathology.

Soo Min Lee1, Yong Ho Choi1, Kyoung Soo Jang1, Hun Kim1, Seon-Woo Lee2, and Gyung Ja Choi1*

1Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, Korea
2Department of Applied Bioscience, Dong-A University, Busan 49315, Korea
Tel: +82-42-860-7434
Fax: +82-42-861-4913
E-mail: kjchoi@krict.re.kr
Received August 2, 2018; Revised August 22, 2018; Accepted August 22, 2018.
cc This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Pectobacterium carotovorum subsp. carotovorum (Pcc) causes bacterial soft rot on a wide range of crops worldwide, especially in countries with warm and humid climates. This study was conducted to establish an efficient screening method for resistant cultivars of radish (Raphanus sativus) to bacterial soft rot. Resistance degrees of 60 commercial radish cultivars to the Pcc KACC 10421 isolate were investigated. For further study, six radish cultivars (Awooriwoldong, YR Championyeolmu, Jeonmuhumu, Bitgoeunyeolmu, Sunbongaltari, Housecheongok) showing different level of resistance to the bacterium were selected. The development of bacterial soft rot on the cultivars was tested according to several conditions such as incubation temperature, seedling stage of radish, inoculum concentration to develop the disease. On the basis of the results, we suggest that an efficient screening method for resistant radish to Pcc is to inoculate twenty-day-old seedlings with a bacterial suspension of Pcc at a concentration of 8×105 cfu/ml and then to cultivate the plants in a growth room at 25°C and 80% RH with 12-hour light per day.
Keywords : Breeding, Disease resistance, Pectobacterium carotovorum subsp. carotovorum, Raphanus sativus, Screening


September 2018, 24 (3)