Research in Plant Disease

Table. 2.

Table. 2.

List of sequences used as markers for avirulence genes

GenePrimerSequence (5’→3’)Tm (°C)aReference
Avr-Pi9Avr-Pi9_F1ATGCAGTTCTCTCAGATCCTC55Selisana et al. (2017)
Avr-Pi9_R2CTACCAGTGCGTCTTTTCGAC
Avr-Pita1Avr-Pita1_F1GCCGAGTCGTTCTGA56Shi et al. (2018)
Avr-Pita1_R2TGTTAATTGTGCAGAAGTTTTT
Avr-Pita2Avr-Pita2_F1TTGGCACCTTTTCATACCCAGTTT60Shi et al. (2018)
Avr-Pita2_R2CAACTTACTTGTGAATCCCATCCC
Avr-Pita3Avr-Pita3_F1ACCGACCCAGGAAAAAAG61Shi et al. (2018)
Avr-Pita3_R2AAGAAACAGGCAAACGCA
AvrPiz-tAvrPiz-t_F1GTTGCGATTATGATCCGTCG57Selisana et al. (2017)
AvrPiz-t_R2GTACTCTAGCAAACGACCGG
Avr-PikAvr-Pik_F1TCCTGCTGCTAACTCCATTC57Selisana et al. (2017)
Avr-Pik_R1TCAACCAAGCGTAAACCTCG
Avr-PikmAvr-Pikm_F1CTGTGGACTAAGTAGCATGCTTCT62Shi et al. (2018)
Avr-Pikm_R2TAGGCAATCAAGAGAAAGCCAGTA
Avr-PiaAvr-Pia_F1CAGAGAAACGGACTTGGAGG57Selisana et al. (2017)
Avr-Pia_R2GGTATACACGTACGGTAGGG
Avr-PiiAvr-Pii_F1GGTAGATATCCGCTGACTGG57Selisana et al. (2017)
Avr-Pii_R2ACTGTCCGCCGCTCGTTTGG
PWL2PWL2_F1GGTGGCGGGTGGACTAAC60Shi et al. (2018)
PWL2_R2CCTCTTCTCGCTGTTCACGG
ACE1ACE1_F1GTTTATCTACGAGGCTGGGGACATT62Shi et al. (2018)
ACE1_R2GGCGAACGGTAAAATGTAGAAGA

Annealing temperature.

Res. Plant Dis. 2019;25:164-72 https://doi.org/10.5423/RPD.2019.25.4.164
© 2019 Res. Plant Dis.