Research in Plant Disease

Fig. 5.

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Fig. 5.

Engineering plants for virus resistance with CRISPR/Cas9 system. Components of the CRISPR/Cas9 machinary, gRNA, and Cas9, are expressed from the plant genome and form gRNA-Cas9 complex. Upon viral infection, the viral DNA replicates through the dsDNA replicative form inside the nucleus of plant cell. The gRNA-Cas9 complex targets the viral DNA dsDNA at complementary target sites and cleaves the viral genome via double stand breaks (DSBs).

Res. Plant Dis. 2018;24:9-25
© 2018 Res. Plant Dis.