Research in Plant Disease

Table. 3.

Table. 3.

Sequence-specific nucleases designed to engineer virus resistance

Nuclease Virus Plant Target References
Zinc finger nuclease (ZFN) TYLCCNV and TbCSV Nicotiana benthamiana Rep Chen et al., 2014
TYLCV Arabidopsis thaliana Rep binding site in IR Mori et al., 2013
TYLCV A. thaliana Rep binding site in IR Koshino-Kimura et al., 2009
TYLCV A. thaliana Rep binding site in IR Koshino-Kimura et al., 2008
TYLCV A. thaliana Rep binding site in IR Takenaka et al., 2007
BSCTV A. thaliana Rep binding site in IR Sera, 2005
Transcription activator-like effector nuclease (TALEN) TbCSV, TLCYnV, TYLCCNV, and TYLCCNB N. benthamiana Rep and Hairpin in IR Cheng et al., 2015
Clustered regularlyinterspaced palindromic TYLCV, BCTV, and MeMV N. benthamiana IR, CP, and Rep Ali et al., 2015
BeYDV N. benthamiana LIR and Rep/RepA Baltes et al., 2015
repeats/CRISPR-associated9 (CRISPR/Cas9) BSCTV N. benthamiana and A. thaliana IR, CP, and Rep Ji et al., 2015
CLCuKoV, TYLCV 2.3,TYLCSV, MeMV,BCTV-Logan,BCTV-Worland N. benthamiana IR, CP, and Rep Ali et al., 2016
TuMV A. thaliana Host factor eIF(iso)4E Pyott et al., 2016
CVYV, ZYMV, and PRSMV Cucumis sativus Host factor eIF4E Chandrasekaran et al., 2016
Res. Plant Dis. 2018;24:9-25 https://doi.org/10.5423/RPD.2018.24.1.9
© 2018 Res. Plant Dis.